sacA [Mon Aug 31 2015 13:57:26 GMT+0200 (CEST)]

phosphosucrase

locus: BSU38040

pI: 5.34

mw: 54.00 kDa

function: utilization of sucrose

product: phosphosucrase

essential: no

ec: 3.2.1.26

synonyms: ipa-50d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]

syns: ipa-50d

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|SacT regulon]

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 32 family (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|sacP]-[protein|search|sacA]-[protein|search|ywdA]'' [Pubmed|3100393]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8702561]

Regulation

• induced by sucrose ([protein|search|SacT]) [Pubmed|2163394]
• repressed by glucose ([protein|search|CcpA])

Regulatory mechanism

• [protein|search|SacT]: binding to a [SW|RNA switch] results in transcription antitermination [Pubmed|2163394,8702561]
• [protein|search|CcpA]: transcription repression

Biological materials

Mutant

• 1A810 ( ''sacA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A810&Search=1A810 BGSC]
• 1A811 ( ''sacA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A811&Search=1A811 BGSC]

References

• 3100393,8702561,2163394