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sacA
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sacA

phosphosucrase
Locus
BSU_38040
Isoelectric point
5.34
Molecular weight
54.72 kDa
Protein length
480 aa Sequence Blast
Gene length
1440 bp Sequence Blast
Function
utilization of sucrose
Product
phosphosucrase
Essential
no
E.C.
3.2.1.26
Synonyms
ipa-50d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of sucrose

    • Gene

    Coordinates
    3,902,210 → 3,903,649

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to UniProt)

    • Protein family

  • glycosyl hydrolase 32 family (with LevB and SacC, according to UniProt)

    • Structure

  • 3PIG (beta-fructofuranosidase from Bifidobacterium longum, 29% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    sacP-sacA-ywdA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • SacT: antitermination, via binding to a RNA switch, in SacT regulon
  • CcpA: repression, in CcpA regulon

    • Regulation

  • induced by sucrose (SacT) PubMed
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    Biological materials

    Mutant

  • 1A810 ( sacA::cat), PubMed, available at BGSC
  • 1A811 ( sacA::kan), PubMed, available at BGSC
  • BKE38040 (ΔsacA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTCATTTTTTCCTCTCCTC, downstream forward: _UP4_TAGAAAATCCTTCTATTTTC
  • BKK38040 (ΔsacA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTCATTTTTTCCTCTCCTC, downstream forward: _UP4_TAGAAAATCCTTCTATTTTC

    • References

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