rpmF

ribosomal protein

locus: BSU_15080

pI: 10.77

mw: 6.59 kDa

protein length: 59 aa

gene length: 180 bp

function: translation

product: ribosomal protein L32

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 1,575,804 1,575,983

The protein

Protein family

• bacterial [SW|ribosomal protein] bL32 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

Expression and Regulation

Operons

• genes: [gene|AF4BB5F08F805D3621597559B4C7F53A1A1CAD05|ylbN]-[gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

Biological materials

Mutant

• BKE15080 ([gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTCCCACCTCCTTAA, downstream forward: _UP4_TAATGAGAAAAGCGCAGGGT
• BKK15080 ([gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTCCCACCTCCTTAA, downstream forward: _UP4_TAATGAGAAAAGCGCAGGGT

References

• 19653700,22383849,11948165,23002217,23033921,25903689