tapA [2017-8-11 16:0:3]

TasA anchoring/assembly protein

Locus: BSU24640

Isoelectric point: 6.68

Molecular weight: 28.92 kDa

Protein length: 253 aa

Gene length: 759 bp

Function: biofilm formation

Product: TasA anchoring/assembly protein

Essential: no

Synonyms: yqhD, yqxM

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Amyloid protein synthesis, secretion and assembly
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,554,486 → 2,555,247

Phenotypes of a mutant

• no biofilm formation PubMed

The protein

Localization

• attached to the cell surface (on the outside of the cell), associated with peptidoglycan PubMed
• secretion requires SipW PubMed

Expression and Regulation

Operons

• Genes: tapA-sipW-tasA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: repression, PubMed, in SinR regulon
  • RemA: activation, PubMed, in RemA regulon
  • LutR: activation, PubMed, in LutR regulon

  Regulation

  • repressed by casamino acids PubMed

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • expression of the operon is localized to a ring near the periphery of the biofilm PubMed

Biological materials

Mutant

• MGNA-C429 (yqhD::erm), available at the NBRP B. subtilis, Japan

LacZ fusion

• pGP1926 (in pAC6), available in Stülke lab

References

Reviews

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Original publications

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