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gamA
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gamA

glucosamine-6-phosphate deaminase
Locus
BSU_02360
Isoelectric point
5.79
Molecular weight
27.14 kDa
Protein length
249 aa Sequence Blast
Gene length
750 bp Sequence Blast
Function
glucosamine utilization
Product
glucosamine-6-phosphate deaminase
Essential
no
Synonyms
ybfT

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
256,823 257,572

Phenotypes of a mutant

  • no growth on glucosamine PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • α-D-glucosamine 6-phosphate + H2O --> β-D-fructose 6-phosphate + NH4+ (according to UniProt)
  • Protein family

  • glucosamine/galactosamine-6-phosphate isomerase family (with NagB, according to UniProt)
  • Paralogous protein(s)

    Kinetic information

  • K(M): 3.0 mM PubMed
  • Structure

  • 2BKX (the structure of NagB, 50% identity) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • GamR: repression, PubMed, in GamR regulon
  • Regulation

  • induced by glucosamine (GamR) PubMed
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    Biological materials

    Mutant

  • MGNA-B938 (ybfT::erm), available at the NBRP B. subtilis, Japan
  • BKE02360 (gamA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACACCCCCTCAAAG, downstream forward: _UP4_TAACACAATAAAAGGAGACT
  • BKK02360 (gamA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGACACCCCCTCAAAG, downstream forward: _UP4_TAACACAATAAAAGGAGACT
  • References

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