SubtiBank SubtiBank
ylnF [2019-06-26 11:14:48]
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ylnF [2019-06-26 11:14:48]

probably precorrin-2 dehydrogenase
Locus
BSU15630
Isoelectric point
9.24
Molecular weight
17.86 kDa
Protein length
162 aa Sequence Blast
Gene length
489 bp Sequence Blast
Function
siroheme biosynthesis , sulfite reduction
Product
probably precorrin-2 dehydrogenase
Essential
no
Synonyms
sirC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme

    • Gene

    Coordinates
    1,635,603 1,636,091

    The protein

    Protein family

  • ferrochelatase family (with HemH, according to UniProt)

    • Structure

  • 3DFZ (36% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    cysH-cysP-sat-cysC-ylnD-sirB-ylnF
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CymR: repression, in CymR regulon
  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

    • Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B370 (ylnF::erm), available at the NBRP B. subtilis, Japan
  • BKE15630 (ylnF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATG
  • BKK15630 (ylnF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATG

    • References

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