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bioF
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bioF

8-amino-7-oxononanoate synthase
locus
BSU_30220
pI
6.28
mw
42.42 kDa
protein length
389 aa Sequence Blast
gene length
1170 bp Sequence Blast
function
biosynthesis of biotin
product
8-amino-7-oxononanoate synthase
essential
no
ec
2.3.1.47
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]

    • Gene

    Coordinates
    3,092,184 3,093,353

    The protein

    Catalyzed reaction/ biological activity

  • pimeloyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2 [pubmed|29054876]

    • Protein family

  • [SW|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|1BC05699DEB3A16944FB8305F22976AED75805E8|Kbl]

    • [SW|Cofactors]

  • PLP (according to UniProt)

    • Structure

  • [PDB|3A2B] (Serine palmitoyltransferase from Sphingobacterium multivorum, 37% identity) [pubmed|19564159]

    • Expression and Regulation

    Operons

    genes
    [gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
    description
    [Pubmed|8763940,8892842]

    regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|8763940,8892842], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]

    • regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
    • view in new tab

    Biological materials

    Mutant

  • BKE30220 ([gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC, downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT
  • BKK30220 ([gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC, downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT

    • References

    Reviews

  • 21437340

    • Original publications

  • 8763940,8892842,21821766,22960854,19564159,29054876