ytxJ

general stress protein, putative bacillredoxin

locus: BSU_29760

pI: 5.09

mw: 12.26 kDa

protein length: 108 aa

gene length: 327 bp

function: unknown

product: putative bacillredoxin

essential: no

synonyms: csb40

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 3,046,757 3,047,083

The protein

Protein family

• Trx family

[SW|Domains]

• contains a single conserved Cys residue in a motif (TCPIS) reminiscent of that found in monothiol glutaredoxins [Pubmed|20308541]

Modification

• phosphorylation on (Thr-93 OR Ser-94 OR Ser-96 OR Thr-99) [Pubmed|17218307]

Structure

• [PDB|3IV4] (from Staphylococcus aureus, 41% identity)

Expression and Regulation

Operons

• genes: [gene|00A42FDB559BE2647CD682180420A435E2E7035F|ytxG]-[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]-[gene|8B4E1D087B75B5E77BCB60FF3465270CEB72B028|ytxJ]
  description: [Pubmed|8733232]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|8733232,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|8733232], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|8733232,11544224]

Biological materials

Mutant

• MGNA-B527 (ytxJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1526 NBRP B. subtilis, Japan]
• BKE29760 ([gene|8B4E1D087B75B5E77BCB60FF3465270CEB72B028|ytxJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAGCTCTTCCTT, downstream forward: _UP4_TAGAAAAAAGCGTCCAGATA
• BKK29760 ([gene|8B4E1D087B75B5E77BCB60FF3465270CEB72B028|ytxJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAGCTCTTCCTT, downstream forward: _UP4_TAGAAAAAAGCGTCCAGATA

References

• 11544224,17218307,8733232,20308541