yxdL

ABC transporter (ATP-binding protein)

Locus: BSU_39640

Isoelectric point: 7.36

Molecular weight: 28.57 kDa

Protein length: 257 aa

Gene length: 774 bp

Function: export of toxic peptides

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Groups of genes → Membrane proteins

Gene

Coordinates: 4,070,393 4,071,166

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• FtsE, BceA, YknY, YtrE, YvrO, PsdA

Domains

• ABC transporter domain (aa 5-243) (according to UniProt)

Structure

• 1L2T (from Methanocaldococcus jannaschii, 43% identity) PubMed

Localization

• plasma membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yxdL-yxdM-yxeA
  Description: PubMed

  Regulatory mechanism

  • YxdJ: activation, PubMed, in YxdJ regulon

  Regulation

  • induced by a cationic antimicrobial peptide, LL-37 (YxdJ) [Pubmed| 21078927]

Biological materials

Mutant

• MGNA-B704 (yxdL::erm), available at the NBRP B. subtilis, Japan
• BKE39640 (yxdL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAAATCCTCCTTATGG, downstream forward: _UP4_CTGTCTATGCTGGGGGGAAA
• BKK39640 (yxdL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAAATCCTCCTTATGG, downstream forward: _UP4_CTGTCTATGCTGGGGGGAAA

References

Loading