ytkL [2019-06-21 11:06:51]

general stress protein

locus: BSU29410

pI: 5.01

mw: 24.68 kDa

protein length: 227 aa

gene length: 684 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 3,009,915 3,010,598

The protein

Protein family

• UPF0173 family (according to Swiss-Prot)

Structure

• [PDB|3X2Z] (from Thermotoga maritima, 49% identity)

Expression and Regulation

Operons

• genes: [gene|3BF3EC6B3139744D968B2FF18CBEEEF15B591AFF|ytkK]-[gene|8B28AA1A88FA56C9F4742F668B28F3323715D948|ytkL]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-A157 (ytkL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/157 NBRP B. subtilis, Japan]
• BKE29410 ([gene|8B28AA1A88FA56C9F4742F668B28F3323715D948|ytkL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCACCTCGTCAC, downstream forward: _UP4_TAAACCGTGAAACCATCTCC
• BKK29410 ([gene|8B28AA1A88FA56C9F4742F668B28F3323715D948|ytkL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTCACCTCGTCAC, downstream forward: _UP4_TAAACCGTGAAACCATCTCC

References

• 15805528,9387221