araP

ABC transporter for the uptake of -1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (integral membrane protein)

Locus: BSU_28740

Isoelectric point: 9.69

Molecular weight: 34.88 kDa

Protein length: 313 aa

Gene length: 942 bp

Function: uptake of -1,5-arabinooligosaccharides

Product: -1,5-arabinooligosaccharide ABC transporter (integral membrane protein)

Essential: no

Synonyms: yseD

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of carbon sources
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,940,697 2,941,638

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• MalFG subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 87-302) (according to UniProt)

Structure

• 2R6G (from E. coli, 23% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

  Regulation

  • induced by arabinose (AraR) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-A986 (araP::erm), available at the NBRP B. subtilis, Japan
• BKE28740 (araP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGAACCTCCCCGCTTT, downstream forward: _UP4_GGCTCGTTTAAGGGGGAGGG
• BKK28740 (araP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGAACCTCCCCGCTTT, downstream forward: _UP4_GGCTCGTTTAAGGGGGAGGG

References

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