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serA
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serA

phosphoglycerate dehydrogenase
Locus
BSU_23070
Isoelectric point
5.62
Molecular weight
56.95 kDa
Protein length
525 aa Sequence Blast
Gene length
1578 bp Sequence Blast
Function
biosynthesis of serine
Product
phosphoglycerate dehydrogenase
Essential
no
E.C.
1.1.1.95
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of serine/ glycine/ alanine
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,411,086 2,412,663

    Phenotypes of a mutant

  • auxotrophic for serine PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • 3-phospho-D-glycerate + NAD+ --> 3-phosphooxypyruvate + H+ + NADH (according to UniProt)
  • (R)-2-hydroxyglutarate + NAD+ --> 2-oxoglutarate + H+ + NADH (according to UniProt)

    • Protein family

  • D-isomer specific 2-hydroxyacid dehydrogenase family (with YvcT and YoaD, according to UniProt)

    • Domains

  • ACT domain (aa 452-524) (according to UniProt)

    • Modification

  • S-cysteinylation after diamide stress (C410)PubMed
  • active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

    • Structure

  • 1YBA (from E. coli, 30% identity, 52% similarity) PubMed

    • Localization

  • membrane PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    serA
    Description
    PubMed

    Regulatory mechanism

  • CcpA: activation, PubMed, in CcpA regulon

    • Regulation

  • activated by CcpA PubMed
  • expression decreases during entry into the stationary phase PubMed
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    Biological materials

    Mutant

  • GP2392 ∆serA::zeo, available in Jörg Stülke's lab PubMed
  • 1A614 (serA::erm), PubMed, available at BGSC and in Jörg Stülke's lab
  • BKE23070 (serA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT
  • BKK23070 (serA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

    • LacZ fusion

  • pGP187 (in pAC7), available in Jörg Stülke's lab
  • pGP2287 (in pAC7), available in Jörg Stülke's lab

    • References

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