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levF [2019-06-06 09:44:54]
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levF [2019-06-06 09:44:54]

fructose-specific permease of the phosphotransferase system, EIIC of the PTS
Locus
BSU27050
Isoelectric point
4.39
Molecular weight
27.87 kDa
Protein length
269 aa Sequence Blast
Gene length
810 bp Sequence Blast
Function
fructose uptake and phosphorylation
Product
fructose-specific PTS, EIIC component
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of fructose
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,761,081 2,761,890

    The protein

    Protein family

  • PTS permease, mannose family PubMed

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    levD-levE-levF-levG-sacC
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • LevR: activation, PubMed, in LevR regulon

    • Regulation

  • induced in the presence of fructose (LevR) PubMed
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    Biological materials

    Mutant

  • BKE27050 (levF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAGACATATTCTCATCCC, downstream forward: _UP4_TAAACGATGAGGGGGAAGAA
  • BKK27050 (levF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAGACATATTCTCATCCC, downstream forward: _UP4_TAAACGATGAGGGGGAAGAA

    • References

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