SubtiBank SubtiBank
levF
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levF

fructose-specific permease of the phosphotransferase system, EIIC of the PTS
Locus
BSU_27050
Isoelectric point
4.39
Molecular weight
27.87 kDa
Protein length
269 aa Sequence Blast
Gene length
810 bp Sequence Blast
Function
fructose uptake and phosphorylation
Product
fructose-specific PTS, EIIC component
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of fructose
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,761,081 2,761,890

    The protein

    Protein family

  • PTS permease, mannose family PubMed

    • Domains

  • PTS EIIC domain type-4 (aa 1-234) (according to UniProt)

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    levD-levE-levF-levG-sacC
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • LevR: activation, PubMed, in LevR regulon

    • Regulation

  • induced in the presence of fructose (LevR) PubMed
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    Biological materials

    Mutant

  • BKE27050 (levF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAGACATATTCTCATCCC, downstream forward: _UP4_TAAACGATGAGGGGGAAGAA
  • BKK27050 (levF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGAAGACATATTCTCATCCC, downstream forward: _UP4_TAAACGATGAGGGGGAAGAA

    • References

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