ysgA [2020-05-14 13:46:50]

similar to rRNA methylase

locus: BSU_28650

pI: 5.18

mw: 26.75 kDa

protein length: 248 aa

gene length: 747 bp

function: unknown

product: unknown

essential: no

ec: 2.1.1.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 2,930,827 2,931,573

Phenotypes of a mutant

• essential [Pubmed|17114254], non-essential according to [Pubmed|28189581]

The protein

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• class IV-like SAM-binding methyltransferase superfamily (with [protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|YacO] and [protein|03BA7AEE17739689D632A35D9C3329EA0982C98A|CspR], according to UniProt)

Paralogous protein(s)

• [protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|YacO]

[SW|Cofactors]

• SAM (according to UniProt)

Structure

• [PDB|4X3M]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|ysgA]
  description: [Pubmed|8969504]

Biological materials

Mutant

• MGNA-B006 (ysgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1005 NBRP B. subtilis, Japan]
• BKE28650 ([gene|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|ysgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC, downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG
• BKK28650 ([gene|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|ysgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC, downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG

References

• 17114254,8969504,28189581