xynD

arabinoxylan arabinofuranohydrolase

locus: BSU_18160

pI: 8.03

mw: 54.33 kDa

protein length: 513 aa

gene length: 1542 bp

function: arabinoxylan degradation

product: arabinoxylan arabinofuranohydrolase

essential: no

ec: 3.2.1.55

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,944,113 1,945,654

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)

Protein family

• [SW|glycosyl hydrolase 43 family] (according to UniProt)

[SW|Domains]

• CBM6 domain (aa 382-511) (accordig to UniProt)

Structure

• [PDB|3C7O] [Pubmed|18980579]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|8A02D56A403BF2E34B84DF0F44347AA2D091D831|xynD]-[gene|55D7F05F8592A53A58A032C78B847782E598B268|xynC]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• BKE18160 ([gene|8A02D56A403BF2E34B84DF0F44347AA2D091D831|xynD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCACATCCTCCTT, downstream forward: _UP4_TAGTACTCGTAATGGGACCG
• BKK18160 ([gene|8A02D56A403BF2E34B84DF0F44347AA2D091D831|xynD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCACATCCTCCTT, downstream forward: _UP4_TAGTACTCGTAATGGGACCG

References

• 18957862,20817675,18980579