abbA [2019-02-19 19:52:42]

inhibitor of AbrB activity

locus: BSU14120

pI: 4.64

mw: 7.56 kDa

protein length: 65 aa

gene length: 198 bp

function: inhibition of AbrB

product: anti-repressor

essential: no

synonyms: ykzF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

Gene

Coordinates: 1,485,118 1,485,315

The protein

Catalyzed reaction/ biological activity

• inhibition of AbrB activity [Pubmed|18840696]

Structure

• [PDB|2LZF] [Pubmed|24534728]

Expression and Regulation

Operons

• genes: [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
  description: [Pubmed|16395550]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in the [SW|Stlke] lab
• BKE14120 ([gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCAGGCCCCCTGTTTTA, downstream forward: _UP4_TAATGGGACACCCTATAATT
• BKK14120 ([gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCAGGCCCCCTGTTTTA, downstream forward: _UP4_TAATGGGACACCCTATAATT

lacZ fusion

• pGP708 (in [protein|search|pAC5]), available in [SW|Stlke] lab, pGP712 (in [protein|search|pAC6]), available in [SW|Stlke] lab

labs

• [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

• 24534728,18840696,14651647,12850135,16395550