SubtiBank SubtiBank
yfmG [2020-04-13 16:38:28]
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yfmG [2020-04-13 16:38:28]

unknown
Locus
BSU_07480
Isoelectric point
5.18
Molecular weight
56.37 kDa
Protein length
487 aa Sequence Blast
Gene length
1464 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
820,867 822,330

The protein

Modification

  • phosphorylated on Arg-474 PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • YfmH: attenuation, PubMed, in YfmH regulon
  • Regulation

  • repressed by glucose (4-fold) PubMed
  • Additional information

  • A ncRNA is predicted between yfmI and yfmG, this has been shown to encode the leader peptide, YfmH PubMed
  • Expression of yfmG is controlled by YfmH-mediated attenuation: slow translation rates of YfmH due to amino acid limitation prevent the formation in the yfmG leader (reduced expression of yfmG if amino acids are available in excess) PubMed
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    Biological materials

    Mutant

  • MGNA-C243 (yfmG::erm), available at the NBRP B. subtilis, Japan
  • BKE07480 (yfmG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATTACGATAACCTCCCG, downstream forward: _UP4_TAATCATTCTAGGTTAGAGA
  • BKK07480 (yfmG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATTACGATAACCTCCCG, downstream forward: _UP4_TAATCATTCTAGGTTAGAGA
  • References

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