yfmG

unknown

locus: BSU_07480

pI: 5.18

mw: 56.37 kDa

protein length: 487 aa

gene length: 1464 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 820,867 822,330

The protein

Modification

• phosphorylated on Arg-474 [Pubmed|22517742]

Structure

• [PDB|2AFT] (human protein, corresponds to aa 205 ... 467, 25% identity) [pubmed|16368756]

Expression and Regulation

Operons

• genes: [gene|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|yfmH]-[gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG]
  description: [Pubmed|32611709]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|32611709], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|YfmH]: attenuation, [pubmed|32611709], in [regulon|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|YfmH regulon]

  regulation

  • repressed by glucose (4-fold) [Pubmed|12850135]

  additional information

  • A [protein|search|ncRNA] is predicted between [gene|3DFC1A118B346C7939851BF711FFA31E04A698E8|yfmI] and [gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG], this has been shown to encode the leader peptide, [protein|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|YfmH] [PubMed|32611709,20525796]
  • Expression of [gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG] is controlled by [protein|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|YfmH]-mediated attenuation: slow translation rates of [protein|8A9E5A121CA2C5CE8A7A2DD2FDCE2AD5FCB356BF|YfmH] due to amino acid limitation prevent the formation in the [gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG] leader (reduced expression of [gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG] if amino acids are available in excess) [pubmed|32611709]

Biological materials

Mutant

• MGNA-C243 (yfmG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2241 NBRP B. subtilis, Japan]
• BKE07480 ([gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTACGATAACCTCCCG, downstream forward: _UP4_TAATCATTCTAGGTTAGAGA
• BKK07480 ([gene|893BB8D1B43F10B46BB7F2DBFE9BE63E9AEEE8EB|yfmG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTACGATAACCTCCCG, downstream forward: _UP4_TAATCATTCTAGGTTAGAGA

References

• 22517742,20817675,21815947,12850135,20525796,16368756,32611709