yfhK

general stress protein, similar to cell division inhibitor

locus: BSU_08570

pI: 9.96

mw: 18.55 kDa

protein length: 172 aa

gene length: 519 bp

function: survival of ethonol stress and at low and high temperatures

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 928,803 929,321

The protein

[SW|Domains]

• 2 [SW|SH3B domain]s (aa 42-105, aa 112-172) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]-[gene|2B7F65B145005A8ADD2A278E3EA5A52DF162C837|yfhL]-[gene|ED478E2C37F2AD8BF06251B52511287EDB91C9EE|yfhM]
  description: [Pubmed|10913081]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10913081,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|10913081,15805528]

Biological materials

Mutant

• MGNA-C317 (yfhK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2315 NBRP B. subtilis, Japan]
• BKE08570 ([gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC, downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT
• BKK08570 ([gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC, downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT

References

• 12107147,15805528,10913081,9987136