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ywnH [2019-08-28 10:45:40]
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ywnH [2019-08-28 10:45:40]

phosphinothricin acetyltransferase
locus
BSU_36560
pI
5.28
mw
18.22 kDa
protein length
163 aa Sequence Blast
gene length
492 bp Sequence Blast
function
unknown
product
phosphinothricin acetyltransferase
essential
no
ec
2.3.1.183
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
  • Gene

    Coordinates
    3,760,694 3,761,185

    The protein

    Catalyzed reaction/ biological activity

  • acetyl-CoA + phosphinothricin --> CoA + H+ + N-acetylphosphinothricin (according to UniProt)
  • Protein family

  • [SW|Acetyltransferase family] (according to UniProt)
  • Structure

  • [PDB|1VHS] [pubmed|16021622]
  • Expression and Regulation

    Operons

    genes
    [gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]-[gene|D09E0307CD931FBAE32B6FF5E19D71A612F0F790|ywnG]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A206 (ywnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/206 NBRP B. subtilis, Japan]
  • BKE36560 ([gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATACGTTTTTGGC, downstream forward: _UP4_TCATGATTAGTCTGGATAAA
  • BKK36560 ([gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATACGTTTTTGGC, downstream forward: _UP4_TCATGATTAGTCTGGATAAA
  • References

  • 27694229,16021622