kdgR [2018-11-23 17:21:42]

transcriptional repressor of the pectin utilization operon (LacI family)

Locus: BSU22120

Isoelectric point: 8.82

Molecular weight: 37.70 kDa

Protein length: 339 aa

Gene length: 1017 bp

Function: regulation of galacturonic acid utilization

Product: transcriptional repressor of the pectin utilization operon (LacI family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of hexuronate
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 2,324,613 → 2,325,632

The protein

Protein family

• LacI family

Structure

• 2HSG (CcpA, 6% identity)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: kdgR-kdgK-kdgA-kdgT
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • KdgR: repression, PubMed, in KdgR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by galacturonate (KdgR) PubMed

Biological materials

Mutant

• BKE22120 (ΔkdgR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGTGCTCCTGTATC, downstream forward: _UP4_TAATCCTAGGTGCCCTCAAC
• BKK22120 (ΔkdgR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGTGCTCCTGTATC, downstream forward: _UP4_TAATCCTAGGTGCCCTCAAC

References

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