hisB

imidazoleglycerol-phosphate dehydratase

Locus: BSU_34900

Isoelectric point: 5.60

Molecular weight: 21.40 kDa

Protein length: 194 aa

Gene length: 585 bp

Function: biosynthesis of histidine

Product: imidazoleglycerol-phosphate dehydratase

Essential: no

E.C.: 4.2.1.19

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of histidine

Gene

Coordinates: 3,585,690 3,586,274

The protein

Catalyzed reaction/ biological activity

• D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate --> 3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O (according to UniProt)

Protein family

• imidazoleglycerol-phosphate dehydratase family (single member, according to UniProt)

Structure

• 2AE8 (from Staphylococcus aureus subsp. aureus n315 mutant, 48% identity, 65% similarity)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI
  Description: PubMed

  Regulatory mechanism

  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional YlxR PubMed

Biological materials

Mutant

• BKE34900 (hisB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCCTGACGCGTTCCGCTT, downstream forward: _UP4_CCGTCAACGAAAGGGATGCT
• BKK34900 (hisB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCCTGACGCGTTCCGCTT, downstream forward: _UP4_CCGTCAACGAAAGGGATGCT

References

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