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pdaA [2018-10-04 10:01:04]
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pdaA [2018-10-04 10:01:04]

N-acetylmuramic acid deacetylase, spore cortex peptidoglycan synthesis
locus
BSU07980
pI
7.32
mw
29.92 kDa
protein length
263 aa Sequence Blast
gene length
789 bp Sequence Blast
function
spore cortex peptidoglycan synthesis
product
N-acetylmuramic acid deacetylase
essential
no
synonyms
yfjS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    869,559 → 870,350

    The protein

    Catalyzed reaction/ biological activity

  • de-N-acetylation of N-acetylglucosamine and N-acetyl-muramic acid for spore cortex peptidoglycan [Pubmed|15687192]

    • Protein family

  • [SW|polysaccharide deacetylase family] (according to UniProt)

    • Structure

  • [PDB|1W17]

    • Expression and Regulation

    Operons

    genes
    [gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]
    description
    [Pubmed|12374835]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|12374835,15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|12374835,15699190]
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    Biological materials

    Mutant

  • MGNA-C273 (yfjS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2271 NBRP B. subtilis, Japan]
  • BKE07980 (Δ[gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG, downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
  • BKK07980 (Δ[gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG, downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC

    • References

  • 12374835,15687192,15699190,15251431,14679227,24405365