yxbA

putative D-aspartate ligase

locus: BSU_39900

pI: 4.43

mw: 10.01 kDa

protein length: 89 aa

gene length: 270 bp

function: unknown

product: putative D-aspartate ligase

essential: no

synonyms: yxaO, aslA

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,098,150 4,098,419

Expression and Regulation

Operons

• genes: [gene|787BB72119DB1321A8281D7C46FED28CAEDBC97C|yxbB]-[gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]-[gene|A15368CD02487EBC1AF999FED34672E38167BCB2|yxnB]
  description: [Pubmed|20185509]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20185509], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by glucose (8-fold) [Pubmed|12850135]

• genes: [gene|787BB72119DB1321A8281D7C46FED28CAEDBC97C|yxbB]-[gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]-[gene|A15368CD02487EBC1AF999FED34672E38167BCB2|yxnB]-[gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]-[gene|BF0E188249161B3CC24D223FDD48B820EBED8F55|yxaM]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20185509], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by glucose (8-fold) [Pubmed|12850135]

Biological materials

Mutant

• MGNA-B763 (yxbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1762 NBRP B. subtilis, Japan]
• BKE39900 ([gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATGCACCTTCCTT, downstream forward: _UP4_AAAGTTAAGCAGGGGGCATG
• BKK39900 ([gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATGCACCTTCCTT, downstream forward: _UP4_AAAGTTAAGCAGGGGGCATG

References

• 10746760,12618455,10498721,20185509,12618455,15101989