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sufS [2019-08-09 09:56:39]
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sufS [2019-08-09 09:56:39]

cysteine desulfurase, cysteine:[protein|search|SufU ]sulfuryl transferase
locus
BSU_32690
pI
5.20
mw
44.76 kDa
protein length
406 aa Sequence Blast
gene length
1221 bp Sequence Blast
function
formation of iron-sulfur clusters in proteins
product
cysteine desulfurase
essential
yes
ec
2.8.1.7
synonyms
yurW,csd
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    3,357,444 3,358,664

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of a CRISPRi knockdown mutant is moderate bulging [Pubmed|27238023]
  • the mutant is viable and Fe-S clusters are dispensable if [protein|search|IspG ]and [protein|search|IspH ]from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate [pubmed|29292548]

    • The protein

    Catalyzed reaction/ biological activity

  • transfer of iron to [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|SufU] [Pubmed|21236255]

    • Protein family

  • [SW|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ]

    • [SW|Cofactors]

  • pyridoxalphosphate [Pubmed|25447671]

    • Effectors of protein activity

  • interaction with [protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|SufU] enhances the activity of [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS] 40-fold [Pubmed|20097860]

    • Structure

  • [PDB|5J8Q] [Pubmed|27382962]
  • [PDB|5XT5] (the [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS]2-[protein|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|SufU]2 complex) [pubmed|29235855]
  • [PDB|5ZS9] (resting state)
  • [PDB|5ZSK] (63 s soak with cysteine)
  • [PDB|5ZSO] (90 s soak with cysteine)

    • Expression and Regulation

    Operons

    genes
    [gene|E966F3808222820C0644DC98F95765B89E6A2551|sufC]-[gene|1A9DA839AB77AF53958CB8426607BEF14F62B85E|sufD]-[gene|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]-[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]-[gene|5991F051C5BAD61A0860BBC64A37DB9D16130998|sufB]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B585 (yurW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1584 NBRP B. subtilis, Japan]
  • BEC32690, a CRISPRi knockdown mutant of ''[gene|C0C30D2A6D3DF7A66063010044D370BD36D27DBF|sufU]'', is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]

    • References

    Reviews

  • 25447671,28882201

    • Original publications

  • 21236255,20097860,20822158,22383849,24321018,15379559,27382962,27238023,29235855,29292548,30855939