sufS

cysteine desulfurase, cysteine:SufU sulfuryl transferase

Locus: BSU_32690

Isoelectric point: 5.20

Molecular weight: 44.76 kDa

Protein length: 406 aa

Gene length: 1221 bp

Function: formation of iron-sulfur clusters in proteins

Product: cysteine desulfurase

Essential: yes

E.C.: 2.8.1.7

Synonyms: yurW,csd

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Iron metabolism → Biosynthesis of iron-sulfur clusters
• Groups of genes → Essential genes

Gene

Coordinates: 3,357,444 3,358,664

Phenotypes of a mutant

• essential PubMed
• terminal phenotype of a CRISPRi knockdown mutant is moderate bulging PubMed
• the mutant is viable and Fe-S clusters are dispensable if IspG and IspH from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate PubMed

The protein

Catalyzed reaction/ biological activity

• transfer of iron to SufU PubMed
• [sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)

Protein family

• Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Paralogous protein(s)

• NifS, YrvO, YcbU, NifZ

Cofactors

• pyridoxalphosphate PubMed

Effectors of protein activity

• interaction with SufU enhances the activity of SufS 40-fold PubMed

Structure

• 5J8Q PubMed
• 5XT5 (the SufS₂-SufU₂ complex) PubMed
• 5ZS9 (resting state)
• 5ZSK (63 s soak with cysteine)
• 5ZSO (90 s soak with cysteine)
• 6KFZ

Expression and Regulation

Operons

• Genes: sufC-sufD-sufS-sufU-sufB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• MGNA-B585 (yurW::erm), available at the NBRP B. subtilis, Japan
• BEC32690, a CRISPRi knockdown mutant of sufU, is available from the Bacillus Genetic Stock Center

References

Reviews

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Original publications

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