nusA

transcription elongation factor of RNA polymerase

Locus: BSU_16600

Isoelectric point: 4.58

Molecular weight: 41.57 kDa

Protein length: 371 aa

Gene length: 1116 bp

Function: transcription

Product: transcription elongation factor

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → Transcription elongation/ termination
• Groups of genes → Essential genes

Gene

Coordinates: 1,732,281 1,733,396

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• stimulates transcription termination at weak intrinsic terminators PubMed

Protein family

• NusA family (single member, according to UniProt)

Domains

• S1 domain (aa 133-199) (according to UniProt)
• KH domain (aa 301-367) (according to UniProt)

Structure

• 1HH2 (from Thermotoga maritima, 51% identity) PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxS-nusA-ylxR-rplGA-infB-ylxP-rbfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • NusA: attenuation, NusA stimulates termination [Reference|http://www.nature.com/articles/nmicrobiol20157], in NusA regulon

  Regulation

  • autoregulation NusA reference
  • induced by glucose PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Reviews

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Original publications

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