cheW [2019-08-28 12:41:28]

modulation of CheA activity in response to attractants, scaffold protein

Locus: BSU_16440

Isoelectric point: 4.42

Molecular weight: 17.36 kDa

Protein length: 156 aa

Gene length: 471 bp

Function: control of CheA activity

Product: CheA modulator

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Coupling proteins

Gene

Coordinates: 1,714,855 1,715,325

Phenotypes of a mutant

• cheV cheW double mutants exhibit complete loss of chemotaxis PubMed

The protein

Paralogous protein(s)

• CheV:

Structure

• 2HO9 (from E. coli, 28% identity)

Localization

• cytoplasm (according to UniProt)
• predominantly present at the cell poles PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • CodY: repression, in CodY regulon
  • DegU: regulation, in DegU regulon
  • SwrA: activation, in SwrA regulon

  Regulation

  • see fla-che operon

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• BKE16440 (cheW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA
• BKK16440 (cheW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA

References

Reviews

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Original publications

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