ylaJ [2019-06-07 10:23:11]

spore lipoprotein

locus: BSU14800

pI: 4.83

mw: 23.24 kDa

protein length: 209 aa

gene length: 630 bp

function: efficient spore [SW|germination]

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,548,681 1,549,310

Phenotypes of a mutant

• reduced rate of spore [SW|germination] in L-alanine [pubmed|28333204]

The protein

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8853C1792A12D9F146FFDC407B6DDD82740EDEA8|ylaJ]
  description: [Pubmed|12480901]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|12480901], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|12480901]

Biological materials

Mutant

• MGNA-A539 (ylaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/539 NBRP B. subtilis, Japan]
• BKE14800 ([gene|8853C1792A12D9F146FFDC407B6DDD82740EDEA8|ylaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGCGGGTTCCCTCCTT, downstream forward: _UP4_TAAGCTATAGCCTTGCGCTT
• BKK14800 ([gene|8853C1792A12D9F146FFDC407B6DDD82740EDEA8|ylaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGCGGGTTCCCTCCTT, downstream forward: _UP4_TAAGCTATAGCCTTGCGCTT

References

• 12107147,12480901,28333204