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gltC
transcriptional activator/ repressor of the
gltA-
gltB operon, activates expression of the operon in the absence of arginine, represses in the presence of glutamate
Molecular weight
33.87 kDa
Function
regulation of the glutamate synthase operon (
gltA-
gltB)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,014,779 2,015,681
Phenotypes of a mutant
gltC mutants are auxotrophic for glutamate, this can be suppressed by the gltR24 mutation or by amplification of the gltA-gltB genomic region PubMed The protein
Catalyzed reaction/ biological activity
Protein family
Domains
DNA-binding helix-turn-helix motif: AA 18 ... 37HTH lysR-type domain (aa 1-58) (according to UniProt) Effectors of protein activity
2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed Structure
2H99 (from Acinetobacter baylyi, corresponds to aa 1 - 245, 35% identity) PubMed Additional information
Expression and Regulation
Biological materials
Mutant
GP344 (erm), (available in Jörg Stülke's lab)GP738 (gltC::Tn10, spc), (available in Jörg Stülke's lab)GP1904 (gltC::aphA3), (available in Jörg Stülke's lab) PubMedBKE18460 (gltC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGCBKK18460 (gltC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC Expression vectors
for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP903, available in Jörg Stülke's labfor expression, purification in E. coli with C-terminal Strep-tag, in pET3C: pGP951, available in Jörg Stülke's lab Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab Antibody
Labs working on this gene/protein
References
Reviews
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Original Publications
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