SubtiBank SubtiBank
gltC
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gltC

transcriptional activator/ repressor of the gltA-gltB operon, activates expression of the operon in the absence of arginine, represses in the presence of glutamate
Locus
BSU_18460
Isoelectric point
5.62
Molecular weight
33.87 kDa
Protein length
300 aa Sequence Blast
Gene length
903 bp Sequence Blast
Function
regulation of the glutamate synthase operon (gltA-gltB)
Product
transcriptional regulator (LysR family)
Essential
No
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,014,779 2,015,681

Phenotypes of a mutant

  • gltC mutants are auxotrophic for glutamate, this can be suppressed by the gltR24 mutation or by amplification of the gltA-gltB genomic region PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the gltA-gltB operon PubMed
  • Protein family

  • LysR family (according to UniProt) PubMed
  • Domains

  • DNA-binding helix-turn-helix motif: AA 18 ... 37
  • HTH lysR-type domain (aa 1-58) (according to UniProt)
  • Effectors of protein activity

  • 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed
  • Structure

  • 2H99 (from Acinetobacter baylyi, corresponds to aa 1 - 245, 35% identity) PubMed
  • Additional information

  • information on binding sites can be found in the PRODORIC2 database
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • GltC: auto-repression, PubMed, in GltC regulon
  • Regulation

  • autoregulation by GltC PubMed
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    Biological materials

    Mutant

  • GP344 (erm), (available in Jörg Stülke's lab)
  • GP738 (gltC::Tn10, spc), (available in Jörg Stülke's lab)
  • GP1904 (gltC::aphA3), (available in Jörg Stülke's lab) PubMed
  • BKE18460 (gltC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
  • BKK18460 (gltC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACATCCAT, downstream forward: _UP4_TAAAAAAAATGAACCCGAGC
  • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP903, available in Jörg Stülke's lab
  • for expression, purification in E. coli with C-terminal Strep-tag, in pET3C: pGP951, available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody

  • available in Jörg Stülke's lab
  • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • Jörg Stülke, University of Göttingen, Germany Homepage
  • Fabian Commichau Senftenberg, Germany homepage
  • References

    Reviews

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    Original Publications

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