pgdS

gamma-DL-glutamyl hydrolase

Locus: BSU_35860

Isoelectric point: 8.52

Molecular weight: 45.09 kDa

Protein length: 413 aa

Gene length: 1242 bp

Function: polyglutamic acid degradation

Product: gamma-DL-glutamyl hydrolase

Essential: no

Synonyms: ywtD

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Capsule biosynthesis and degradation
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,696,257 3,697,498

The protein

Protein family

• Peptidase C40 family (according to UniProt)

Domains

• three NlpC/P60 domains (only the central domain is active) PubMed

Structure

• 4HPE (from Clostridium difficile, 39% identity)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: pgdS
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

Biological materials

Mutant

• MGNA-A545 (ywtD::erm), available at the NBRP B. subtilis, Japan
• BKE35860 (pgdS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTATTATCTCCTCCT, downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT
• BKK35860 (pgdS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTTATTATCTCCTCCT, downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT

LacZ fusion

• available in Daniel Kearns's lab PubMed

References

Reviews

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Original publications

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