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clpC [2017-05-02 13:23:13]
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clpC [2017-05-02 13:23:13]

AAA unfoldase, ATPase subunit of the ClpC-ClpP protease, directs proteins phosphorylated on arginine residues to ClpP
locus
BSU00860
pI
5.75
mw
89.93 kDa
protein length
810 aa Sequence Blast
gene length
2430 bp Sequence Blast
function
protein degradationpositive regulator of autolysin (LytC and LytD) synthesis
product
AAA unfoldase, ATPase subunit of the ClpC-ClpP protease
essential
no
synonyms
mecB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon], [SW|SigF regulon]

    • Gene

    Coordinates
    103,572 → 106,004

    Phenotypes of a mutant

  • inactivation of [gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] reduces sporulation efficiency to 0.4% that of wild type cells; delayed entry into sporulation, defect in engulfment with reduced SigG activity, and production of small spores [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • [gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA] family (according to Swiss-Prot) clpA/clpB family. [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] subfamily (according to Swiss-Prot), AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Paralogous protein(s)

  • [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|ClpE], [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|ClpX]

    • [SW|Domains]

  • AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Modification

  • phosphorylated on Arg-5 and Arg-254 [Pubmed|22517742]

    • Structure

  • [PDB|2K77] (N-terminal domain)
  • [PDB|3PXG], [PDB|3J3U 3J3U] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|MecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] complex) [Pubmed|23595989]

    • [SW|Localization]

  • cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
  • forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
  • [[File:ClpC.jpg‎ ]]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
    description
    [Pubmed|8793870]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8793870], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30962353], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • expressed during germination and spore outgrowth [Pubmed|24244006]
  • induction during diamide stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30962353]
    • view in new tab

    Biological materials

    Mutant

  • [gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
  • BP98 (''clpC''::''spc''), available in [SW|Fabian Commichau]'s lab [Pubmed|25610436]
  • BKE00860 (''clpC''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]

    • GFP fusion

  • C-terminal GFP fusions (single copy, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

    • Antibody

  • available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs

    • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
  • [SW|Kürsad Turgay], Freie Universität Berlin, Germany [http://www.biologie.fu-berlin.de/en/microbio/mibi-turg/index.html homepage]

    • References

    Reviews

  • 17302811,23375660,23479438,19609260,19781636,26639779

    • Original Publications

  • 9987115,8016067,9000055,12923101,10447896,9141693,2113920,16497325,19226326,8793870,10809708,14679237,17560370,11684022,8195092,11722737,11914365,12028382,18689476,19361434,9890793,19767395,9987115,11544224,17981983,14763982,8016066,19361434,18689473,20070525,20923420,20852588,22517742,21622759,21368759,21821766,23595989,18786145,16525504,17380125,16163393,12598648,24263382,25610436,26458230,26735940,27014237,27749819