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clpC [Mon Oct 24 2016 11:26:51 GMT+0200 (CEST)]
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clpC [Mon Oct 24 2016 11:26:51 GMT+0200 (CEST)]

AAA unfoldase, ATPase subunit of the ClpC-ClpP protease
locus
BSU00860
pI
5.00
mw
89.00 kDa
function
protein degradation
positive regulator of autolysin (LytC and LytD) synthesis
product
AAA unfoldase, ATPase subunit of the ClpC-ClpP protease
essential
no
synonyms
mecB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|proteolysis], [SW|sporulation proteins], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon], [SW|SigF regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    103,572 -> 106,004

    Phenotypes of a mutant

  • inactivation of ''[SW|clpC]'' reduces sporulation efficiency to 0.4% that of wild type cells; delayed entry into sporulation, defect in engulfment with reduced SigG activity, and production of small spores [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • [protein|search|mecA] family (according to Swiss-Prot) clpA/clpB family. [protein|search|ClpC] subfamily (according to Swiss-Prot), AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Paralogous protein(s)

  • [protein|search|ClpE], [protein|search|ClpX]

    • [SW|Domains]

  • AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Modification

  • phosphorylated on Arg-5 and Arg-254 [Pubmed|22517742]

    • Structure

  • [PDB|2K77] (N-terminal domain)
  • [PDB|3PXG], [PDB|3J3U 3J3U] (the [protein|search|MecA]-[protein|search|ClpC] complex) [Pubmed|23595989]

    • [SW|Localization]

  • cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with [protein|search|ClpP] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
  • forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
  • [[File:ClpC.jpg‎ ]]

    • [SW|Interactions]

  • forms hexamers [Pubmed|26639779]
  • the protease: [protein|search|ClpC]-[protein|search|ClpP]
  • interactions with adaptor proteins of [protein|search|ClpC]-[protein|search|ClpP]: [protein|search|YpbH]-[protein|search|ClpC], [protein|search|McsB]-[protein|search|ClpC], [protein|search|MecA]-[protein|search|ClpC] [Pubmed|9000055,9890793,10447896,11914365,12598648,16525504,17380125]
  • [protein|search|CtsR]-[protein|search|McsB]-[protein|search|ClpC]/[protein|search|ClpP] (degradation of [protein|search|CtsR]) [Pubmed|11179229,16163393,17380125,20852588]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

    • Expression and Regulation

    Operon

  • ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [protein|search|SigF] [Pubmed|16497325]

    • Regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]
  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]
  • induced by heat ([protein|search|CtsR]) [Pubmed|9987115]

    • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed1], [http://www.ncbi.nlm.nih.gov/pubmed/11179229 PubMed2], [http://www.ncbi.nlm.nih.gov/pubmed/16163393 PubMed3], [http://www.ncbi.nlm.nih.gov/pubmed/17380125 PubMed4]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

    • Biological materials

    Mutant

  • ''[protein|search|clpC]::tet'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
  • BP98 (''clpC''::''spc''), available in [SW|Fabian Commichau]'s lab [Pubmed|25610436]
  • BKE00860 (''clpC''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]

    • GFP fusion

  • C-terminal GFP fusions (single copy, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab

    • Antibody

  • available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs

    • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
  • [SW|Kürsad Turgay], Freie Universität Berlin, Germany [http://www.biologie.fu-berlin.de/en/microbio/mibi-turg/index.html homepage]

    • References

    Reviews

  • 17302811,23375660,23479438,19609260,19781636,26639779

    • Original Publications

  • 9987115,8016067,9000055,12923101,10447896,9141693,2113920,16497325,19226326,8793870,10809708,14679237,17560370,11684022,8195092,11722737,11914365,12028382,18689476,19361434,9890793,19767395,9987115,11544224,17981983,14763982,8016066,19361434,18689473,20070525,20923420,20852588,22517742,21622759,21368759,21821766,23595989,18786145,16525504,17380125,16163393,12598648,24263382,25610436,26458230,26735940,27014237