ohrA [2018-08-17 12:20:55]

peroxiredoxin , protects the cell against organic peroxides

locus: BSU13140

pI: 5.06

mw: 14.73 kDa

protein length: 141 aa

gene length: 423 bp

function: organic peroxide resistance

product: peroxiredoxin

essential: no

synonyms: yklA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 1,380,978 → 1,381,403

The protein

Protein family

• osmC/ohr family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]
  description: [Pubmed|9696771]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9696771], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7A3A8AF7728474204396BB7A548F747D28E6FAC7|OhrR]: repression, [Pubmed|11418552], in [regulon|7A3A8AF7728474204396BB7A548F747D28E6FAC7|OhrR regulon]

  regulation

  • induced by organic peroxides ([protein|search|OhrR]) [Pubmed|11418552]

Biological materials

Mutant

• MGNA-A749 (yklA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/749 NBRP B. subtilis, Japan]
• BKE13140 (Δ[gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATTCCCCCAATA, downstream forward: _UP4_TAAATAAAAAGAGGATGCCT
• BKK13140 (Δ[gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATTCCCCCAATA, downstream forward: _UP4_TAAATAAAAAGAGGATGCCT

References

• 16209951,12486061,11418552,9696771