yceE

general stress protein, similar to tellurium resistance protein

locus: BSU_02910

pI: 4.39

mw: 20.80 kDa

protein length: 192 aa

gene length: 579 bp

function: required for survival of ethanol stress and at low temperatures

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.12|Resistance against toxic metals/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 313,396 313,974

The protein

Protein family

• CAPAB/TerDEXZ family (with [protein|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|YceC] and [protein|DF30A8B195B8D830E131B23C768F07BE61F51150|YceD], according to UniProt)

Paralogous protein(s)

• [protein|DF30A8B195B8D830E131B23C768F07BE61F51150|YceD], [protein|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|YceC]

Modification

• phosphorylated on Arg-10 [Pubmed|22517742]

Structure

• [PDB|2KXT] (from Klebsiella pneumoniae, 54% identity) [pubmed|21112337]

Expression and Regulation

Operons

• genes: [gene|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|yceC]-[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]-[gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]-[gene|F16A11027D8D320D3D64E44038E29921A946955B|yceF]-[gene|F588108F12C5CA855A24159F28223EF67145D28F|yceG]-[gene|3A9EB32D5627FAB3F7646B7918E54C8D1ADD25DF|yceH]
  description: [Pubmed|11866510]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|19047346], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|7E4742F30B4C43FBF9DAE0B02948022AE7912A33|yceC]-[gene|DF30A8B195B8D830E131B23C768F07BE61F51150|yceD]-[gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]-[gene|F16A11027D8D320D3D64E44038E29921A946955B|yceF]
  description: [Pubmed|11866510]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|11544224,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [pubmed|19047346], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• MGNA-B985 (yceE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1984 NBRP B. subtilis, Japan]
• BKE02910 ([gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGCCTCTCTCCTTTC, downstream forward: _UP4_TAAGCGAGTGACAAATAGAG
• BKK02910 ([gene|863E26D497D29B820912A0C0F4A3AE4C6865EC2E|yceE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGCCTCTCTCCTTTC, downstream forward: _UP4_TAAGCGAGTGACAAATAGAG

References

• 15805528,11866510,18179421,19047346,22517742,21112337