dltC [2018-10-05 10:02:40]

D-alanine carrier protein, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides

Locus: BSU38520

Isoelectric point: 3.62

Molecular weight: 8.87 kDa

Protein length: 78 aa

Gene length: 234 bp

Function: biosynthesis of teichoic acid

Product: D-alanine carrier protein

Essential: no

E.C.: 6.1.1.13

Synonyms: ipa-3r

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of teichoic acid
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of teichoic acid
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Gene

Coordinates: 3,954,987 → 3,955,223

Phenotypes of a mutant

• more sensitive to nisin PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) (according to Swiss-Prot)

Modification

• modified with the 4′-phosphopantetheine (Ppant) group at Ser35, by AcpS PubMed

Structure

• 4BPG PubMed

Expression and Regulation

Operons

• Genes: ywzH-dltA-dltB-dltC-dltD-dltE
  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
  • SigM: sigma factor, PubMed, in SigM regulon
  • SigX: sigma factor, PubMed, in SigX regulon
  • SigV: sigma factor, PubMed, in SigV regulon

  Regulatory mechanism

  • YvrHb: activation, PubMed, in YvrHb regulon
  • Spo0A: repression, PubMed, in Spo0A regulon
  • stringent response: negative regulation, in stringent response

  Regulation

  • expression is reduced in a SigV mutant PubMed
  • the mRNA is processed between ywzH and dltA by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Mutant

• BKE38520 (ΔdltC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT, downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA
• BKK38520 (ΔdltC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT, downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA

References

Reviews

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Original publications

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