pksF [2019-08-01 13:22:36]

involved in polyketide synthesis

Locus: BSU_17140

Isoelectric point: 5.37

Molecular weight: 44.97 kDa

Protein length: 416 aa

Gene length: 1248 bp

Function: polyketide synthesis

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 1,788,695 → 1,789,942

The protein

Protein family

• thiolase-like superfamily (according to UniProt)

Structure

• 4LS5 (FabF, 30% identity) PubMed

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • CodY: activation, PubMed, in CodY regulon

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
  Description: PubMed

Biological materials

Mutant

• BKE17140 (ΔpksF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGCCTACACCGGTCACCA, downstream forward: _UP4_GAGAAATGTGGAGGCGAATC
• BKK17140 (ΔpksF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACGCCTACACCGGTCACCA, downstream forward: _UP4_GAGAAATGTGGAGGCGAATC

References

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