ytpA

phospholipase (annotated as a bacilysocin synthase)

locus: BSU_30510

pI: 6.77

mw: 29.42 kDa

protein length: 259 aa

gene length: 780 bp

function: lipid degradation

product: phospholipase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,122,662 3,123,441

The protein

Catalyzed reaction/ biological activity

• cleaves fatty acids from the 2 position of phosphatidylglycerol [Pubmed|11796336]

Protein family

• [SW|AB hydrolase superfamily] (according to UniProt)

[SW|Domains]

• 3 [SW|AB hydrolase-1 domain]s (aa 37-52, aa 84-97, aa 198-212) (according to InterPro)

Structure

• [PDB|3HJU] (human monoglyceride lipase, 30% identity) [Pubmed|19957260]

Expression and Regulation

Operons

• genes: [gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]-[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
  description: [Pubmed|18179421]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]

Biological materials

Mutant

• MGNA-A128 (ytpA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/128 NBRP B. subtilis, Japan]
• BKE30510 ([gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCATACG, downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT
• BKK30510 ([gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCATACG, downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT

References

• 18179421,11796336,19957260