tnrA

transcriptional pleiotropic regulator ([SW|MerR family]) involved in global nitrogen regulation

locus: BSU_13310

pI: 10.23

mw: 12.99 kDa

protein length: 110 aa

gene length: 333 bp

function: regulation of nitrogen assimilation

product: transcription activator/ repressor ([SW|MerR family])

essential: no

synonyms: scgR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

Gene

Coordinates: 1,397,411 1,397,743

The protein

Protein family

• [SW|MerR family]

[SW|Domains]

• [SW|HTH merR-type domain] (aa 13-81) (according to UniProt)

Effectors of protein activity

• feedback-inhibited [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|GlnA] prevents [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA] from DNA binding

Structure

• [PDB|4R22] (the TnrA-DNA complex) [Pubmed|25691471]
• [http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]-[protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|GlnA] complex) [Pubmed|25691471]

[SW|Localization]

• membrane-associated via [protein|796BD46066B1E6147048664FA78679B42FA9D628|NrgA]-[protein|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|NrgB] under conditions of poor nitrogen supply [Pubmed|21435182]

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000026 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [PubMed|10671441,16547045], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR]: repression, in [regulon|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]) [Pubmed|10671441,16547045]

Biological materials

Mutant

• GP252 (in frame deletion), available in [SW|Jörg Stülke]'s lab
• BKE13310 (''tnrA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• GP2262 (''tnrA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
• 1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]
• 1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]
• BKE13310 ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
• BKK13310 ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

Expression vectors

• for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP171 available in [SW|Jörg Stülke]'s lab
• pGP229 (N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in the [SW|Karl Forchhammer] lab

labs

• [SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
• [SW|Maria Schumacher], Durham, NC, USA [http://www.dukeschumacherlab.com/index.html homepage]

References

Reviews

• 22625175,10231480,18086213

The [SW|TnrA regulon]

• 12823818,25755103

Control of TnrA activity by the [SW|trigger enzymes|trigger enzyme] [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|GlnA]

• 11719184,12139611,17085574,19233925,16885465,26635369

Other original publications

• 12374841,9287005,12950915,10671441,16547045,8799114,15150225,11029411,17001076,15547269,2573733,8636055,16493705,6141156,18667567,21435182,23535029,23808168,25157083,25691471
• [Pubmed|]