tnrA

transcriptional pleiotropic regulator (MerR family) involved in global nitrogen regulation

Locus: BSU_13310

Isoelectric point: 10.23

Molecular weight: 12.99 kDa

Protein length: 110 aa

Gene length: 333 bp

Function: regulation of nitrogen assimilation

Product: transcription activator/ repressor (MerR family)

Essential: no

Synonyms: scgR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Information processing → Regulation of gene expression → Regulators of core metabolism

Gene

Coordinates: 1,397,411 1,397,743

The protein

Protein family

• MerR family

Domains

• HTH merR-type domain (aa 13-81) (according to UniProt)

Effectors of protein activity

• feedback-inhibited GlnA prevents TnrA from DNA binding

Structure

• 4R22 (the TnrA-DNA complex) PubMed
• 4S0R (the TnrA-GlnA complex) PubMed

Localization

• membrane-associated via NrgA-NrgB under conditions of poor nitrogen supply PubMed

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: tnrA
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • GlnR: repression, in GlnR regulon

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

Biological materials

Mutant

• GP252 (in frame deletion), available in Jörg Stülke's lab
• BKE13310 (tnrA::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
• GP2262 (tnrA::ermC) (available in Jörg Stülke's lab)
• 1A851 ( tnrA::erm), PubMed, available at BGSC
• 1A851 ( tnrA::erm), PubMed, available at BGSC
• BKE13310 (tnrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
• BKK13310 (tnrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP171 available in Jörg Stülke's lab
• pGP229 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab

Antibody

• available in the Karl Forchhammer lab

Labs working on this gene/protein

• Susan Fisher, Boston, USA homepage
• Maria Schumacher, Durham, NC, USA homepage

References

Reviews

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The TnrA regulon

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Control of TnrA activity by the trigger enzyme GlnA

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Other original publications

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