dppE

dipeptide ABC transporter (dipeptide-binding protein)

Locus: BSU_12960

Isoelectric point: 5.52

Molecular weight: 62.41 kDa

Protein length: 549 aa

Gene length: 1650 bp

Function: uptake of dipeptides

Product: dipeptide ABC transporter (dipeptide-binding protein)

Essential: no

Synonyms: dciAE

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,364,151 1,365,800

The protein

Protein family

• bacterial solute-binding protein 5 family (according to UniProt)

Paralogous protein(s)

• OppA

Structure

• 4FAJ (from Enterococcus faecalis, 33% identity) PubMed

Localization

• attached to the cell membrane (via DppB-DppC) PubMed

Expression and Regulation

Operons

• Genes: dppA-dppB-dppC-dppD-dppE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by glucose (2.9-fold) PubMed

• Genes: dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD
  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

Biological materials

Mutant

• GP1110 (spc), available in Jörg Stülke's lab
• 1A737 ( dppE::kan), PubMed, available at BGSC
• BKE12960 (dppE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTCTTCCCCCTTTTCA, downstream forward: _UP4_TGATGGAGGCGATTGAGGAA
• BKK12960 (dppE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTCTTCCCCCTTTTCA, downstream forward: _UP4_TGATGGAGGCGATTGAGGAA

References

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