rsbX [2020-10-13 17:30:44]

protein serine phosphatase, feedback PP2C, dephosphorylates RsbS and RsbR

locus: BSU_04740

pI: 6.23

mw: 22.00 kDa

protein length: 199 aa

gene length: 600 bp

function: control of SigB activity

product: protein serine phosphatase, feedback PP2C

essential: no

ec: 3.1.3.3

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 523,650 524,249

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR]-P [Pubmed|15466036] and [protein|841CE7FCA4E84445830CA18F9856F6F30014E3BB|RsbS]-S59-P [Pubmed|8824586,21362065]
• H₂O + O-phospho-L-(or D)serine --> L-(or D)serine + phosphate (according to UniProt)

[SW|Domains]

• [SW|PPM-type phosphatase domain] (aa 11-198) (according to UniProt)

Structure

• [PDB|3W42], [PDB|3W43] [Pubmed|26057679]

Expression and Regulation

Operons

• genes: [gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
  description: [Pubmed|8002610]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002610], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|20454630], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
  description: [Pubmed|2170324]

  regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• BKE04740 ([gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCGTTTTCTTCAACCTGGA, downstream forward: _UP4_TAAAAAACCAGAAAAAGAAG
• BKK04740 ([gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCGTTTTCTTCAACCTGGA, downstream forward: _UP4_TAAAAAACCAGAAAAAGAAG

labs

• [SW|Bill Haldenwang], San Antonio, USA
• [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

References

• 8002610,8682769,9658013,8002609,10671474,9068644,19923733,11544224,15466036,8824586,23407164,23320651,21362065,26057679