ylbB

putative oxidoreductase

locus: BSU_14950

pI: 4.76

mw: 16.21 kDa

protein length: 148 aa

gene length: 447 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,565,849 1,566,295

The protein

Paralogous protein(s)

• [protein|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|YhcV]:

[SW|Domains]

• 2 [SW|CBS domain]s (aa 8-68, aa 74-130) (according to UniProt)

Structure

• [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 38% identity) [Pubmed|23382856]

Expression and Regulation

Operons

• genes: [gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]-[gene|2EC813B89149AD39405E66C325FB2684B5987162|ylbC]
  description: [Pubmed|16497325]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,15699190]

Biological materials

Mutant

• MGNA-B242 (ylbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1241 NBRP B. subtilis, Japan]
• BKE14950 ([gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA
• BKK14950 ([gene|8529D83560E934709B20CCF0B72251A74EC272BB|ylbB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA

Expression vectors

• pGP2934 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 16497325,15699190,23382856