ctpB [2018-07-16 12:37:54]

carboxy-terminal processing serine protease, cleaves [protein|AB2A3422277040107AAF90358BBE58608F8E0738|SpoIVFA], this results in processing of pro-[protein|search|SigK]

locus: BSU35240

pI: 8.64

mw: 52.63 kDa

protein length: 480 aa

gene length: 1440 bp

function: control of [protein|search|SigK] activation

product: carboxy-terminal processing serine protease, cleaves [protein|AB2A3422277040107AAF90358BBE58608F8E0738|SpoIVFA]

essential: no

synonyms: yvjB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,622,356 → 3,623,798

The protein

Protein family

• PDZ protease [Pubmed|24243021]

Paralogous protein(s)

• [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA]

[SW|Domains]

• signal peptide (aa 1 - 23) (according to Uniprot)
• [SW|PDZ domain] (aa 92 - 182) (according to Uniprot)
• S41 peptidase domain (by similarity to [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA]) [pubmed|29979679]
• peptidoglycan binding domain (C-terminal) (by similarity to [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA]) [pubmed|29979679]

Effectors of protein activity

• substrate binding of CtpB induces opening of the PDZ gate and protease activation [Pubmed|24243021]
• [protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|CtpB] acts in concert with a second signaling protease, [protein|DBB3ED60A7D162B9F2830F81041BC661AA5EC1E7|SpoIVB] [Pubmed|24243021]

Structure

• [PDB|4C2C] [Pubmed|24243021]
• forms a ring-like scaffold with two narrow tunnels for substrate binding [Pubmed|24243021]

[SW|Localization]

• intracellular space between the mother cell and the forespore [Pubmed|24243021]

Expression and Regulation

Operons

• genes: [gene|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,16497325], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE], [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]) [Pubmed|15699190,16497325]

Biological materials

Mutant

• MGNA-A074 (yvjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/74 NBRP B. subtilis, Japan]
• 1S129 ( ''ctpB''::''tet''), [Pubmed|14526016], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S129&Search=1S129 BGSC]
• BKE35240 (Δ[gene|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT
• BKK35240 (Δ[gene|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT

References

• 14526016,17557826,16818230,15699190,16497325,24243021