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obg [2019-02-19 19:52:30]
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obg [2019-02-19 19:52:30]

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit
Locus
BSU27920
Isoelectric point
4.86
Molecular weight
47.53 kDa
Protein length
428 aa Sequence Blast
Gene length
1287 bp Sequence Blast
Function
ribosome assembly, may be required to stimulate activity
Product
GTP-binding protein
Essential
Yes
E.C.
3.6.5.-
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,852,661 2,853,947

Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family

  • GTP1/OBG family (according to Swiss-Prot) Era/Obg family
  • Paralogous protein(s)

    Domains

  • Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
  • Cofactors

  • GDP, GTP and probably ppGpp
  • Structure

    Additional information

  • The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Additional information

  • expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed
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    Biological materials

    Expression vectors

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP835, available in Stlke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP843, available in Stlke lab
  • Labs working on this gene/protein

  • Bill Haldenwang, San Antonio, USA
  • Naotake Ogasawara, Nara, Japan
  • References

    Reviews

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    Original publications

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