obg

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

locus: BSU_27920

pI: 4.86

mw: 47.53 kDa

protein length: 428 aa

gene length: 1287 bp

function: ribosome assembly, may be required to stimulate activity

product: GTP-binding protein

essential: Yes

ec: 3.6.5.-

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

Gene

Coordinates: 2,852,661 2,853,947

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA] and [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF], according to UniProt)

Paralogous protein(s)

• [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|YqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|YphC], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|Era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|YsxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|ThdF]

[SW|Domains]

• Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
• Obg domain (aa 1-158) (according to UniProt)
• OBG-type G domain (aa 159-329) (according to UniProt)
• OCT domain (aa 350-428) (according to UniProt)

[SW|Cofactors]

• GDP, GTP and probably ppGpp

Structure

• [PDB|1LNZ] [pubmed|12429099]

Additional information

• The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.

Expression and Regulation

Operons

• genes: [gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2537815], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  additional information

  • expression is repressed by binding of [SW|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]

Biological materials

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP835, available in [SW|Jörg Stülke]'s lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP843, available in [SW|Jörg Stülke]'s lab

labs

• [SW|Bill Haldenwang], San Antonio, USA
• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 19575570,21885683

Original publications

• 15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966,12427945,12429099,2537815,20830302