obg

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

Locus: BSU_27920

Isoelectric point: 4.86

Molecular weight: 47.53 kDa

Protein length: 428 aa

Gene length: 1287 bp

Function: ribosome assembly, may be required to stimulate activity

Product: GTP-binding protein

Essential: Yes

E.C.: 3.6.5.-

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
• Information processing → Regulation of gene expression → phosphorelay
• Lifestyles → Sporulation → phosphorelay
• Groups of genes → Essential genes
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 2,852,661 2,853,947

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• TRAFAC class OBG-HflX-like GTPase superfamily (with YnbA and YyaF, according to UniProt)

Paralogous protein(s)

• YqeH, YnbA, YphC, YyaF, Era, YsxC, RbgA, ThdF

Domains

• Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
• Obg domain (aa 1-158) (according to UniProt)
• OBG-type G domain (aa 159-329) (according to UniProt)
• OCT domain (aa 350-428) (according to UniProt)

Cofactors

• GDP, GTP and probably ppGpp

Structure

• 1LNZ PubMed

Additional information

• The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and Regulation

Operons

• Genes: spo0B-obg-thrR-pheA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Additional information

  • expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed

Biological materials

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP835, available in Jörg Stülke's lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP843, available in Jörg Stülke's lab

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Naotake Ogasawara, Nara, Japan

References

Reviews

Loading

Original publications

Loading