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fhuD [2019-08-26 10:54:22]
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fhuD [2019-08-26 10:54:22]

hydroxamate siderophore [SW|ABC transporter ](only ferrichrome) (binding protein)
locus
BSU_33320
pI
9.16
mw
34.27 kDa
protein length
315 aa Sequence Blast
gene length
948 bp Sequence Blast
function
siderophore uptake
product
hydroxamate siderophore [SW|ABC transporter ](only ferrichrome) (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    3,418,474 3,419,421

    Phenotypes of a mutant

  • poor growth with the xenosiderophore ferrichrome as single source of iron [Pubmed|23220087]

    • The protein

    Protein family

  • [SW|Bacterial solute-binding protein 8 family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|6F4F7A68701FAC3983AF447F11BC1EAB17D7B07B|YxeB]

    • [SW|Domains]

  • Fe/B12 periplasmic-binding domain (aa 60-315) (according to UniProt)

    • Structure

  • [PDB|4FNA] (from Staphylococcus aureus, 38% identity) [pubmed|24606332]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|fhuD]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
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    Biological materials

    Mutant

  • BKE33320 ([gene|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|fhuD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTCATACCTTCTTTC, downstream forward: _UP4_TAAACAAAAGAGCCGCCTGC
  • BKK33320 ([gene|84CC89907EF39770F5BFACA82A11B04CF76EC5AA|fhuD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTCATACCTTCTTTC, downstream forward: _UP4_TAAACAAAAGAGCCGCCTGC

    • References

  • 10092453,16672620,12354229,18957862,23220087,29133393,24606332