dnaN [2017-12-07 13:27:19]

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization

Locus: BSU00020

Isoelectric point: 4.72

Molecular weight: 41.94 kDa

Protein length: 378 aa

Gene length: 1134 bp

Function: DNA replication, DNA repair

Product: DNA polymerase III (beta subunit), beta clamp

Essential: yes

E.C.: 2.7.7.7

Synonyms: dnaG

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → Genetics → DNA repair/ recombination → Mismatch repair (MMR)
• Groups of genes → Essential genes

Gene

Coordinates: 1,939 → 3,075

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed
• Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
• recruits MutS to the site of DNA replication (replisome) PubMed
• required for bacteriophage SPP1 replication PubMed
• inhibits oligomerization and helix formation of DnaA PubMed

Structure

• 2AWA (from Streptococcus pneumoniae, 41% identity, 77% similarity)

Localization

• nucleoid (mid-cell spot) PubMed PubMed
• forms large assemblies on DNA, called clamp zones PubMed
• forms foci PubMed

Expression and Regulation

Operons

• Genes: dnaA-dnaN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon

  Regulation

  • negatively controlled by DnaA PubMed and Spo0A PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of dnaA PubMed

Biological materials

GFP fusion

• PubMed PubMed

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

Labs working on this gene/protein

• Philippe Noirot, Jouy-en-Josas, France homepage

References

Reviews

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Original publications

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