dnaN [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization

Locus: BSU00020

Isoelectric point: 4.00

Molecular weight: 41.00 kDa

Function: DNA replication, DNA repair

Product: DNA polymerase III
(beta subunit), beta clamp

Essential: yes

E.C.: 2.7.7.7

Synonyms: dnaG

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → Genetics → DNA repair/ recombination → Mismatch repair (MMR)
• Groups of genes → Essential genes

Categories containing this gene/protein

• DNA replication, essential genes

This gene is a member of the following regulons

• Spo0A regulon

Gene

Coordinates on the chromosome (coding sequence): 1,939 -> 3,075

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed
• Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
• recruits MutS to the site of DNA replication (replisome) PubMed
• required for bacteriophage SPP1 replication PubMed
• inhibits oligomerization and helix formation of DnaA PubMed

Structure

• 2AWA (from Streptococcus pneumoniae, 41% identity, 77% similarity)

Localization

• nucleoid (mid-cell spot) PubMed PubMed
• forms large assemblies on DNA, called clamp zones PubMed
• forms foci PubMed

Interactions

• DnaA-YabA-DnaN PubMed
• part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
• PolY1-DnaN, PolY2-DnaN (required for untargeted mutagenesis) PubMed
• PolY1-PolA-DnaN PubMed
• MutL (C-terminal domain)-DnaN, the interaction stimulates the endonuclease activity of MutL PubMed
• MutS-DnaN PubMed
• DnaE-DnaN PubMed
• DnaN forms dimers PubMed

Expression and Regulation

Operon

• dnaA-dnaN PubMed

Sigma factor

• SigA PubMed

Regulation

• negatively controlled by DnaA PubMed and Spo0A PubMed
• repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism

• Spo0A: transcription repression PubMed

Biological materials

GFP fusion

• PubMed PubMed

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

Labs working on this gene/protein

• Philippe Noirot, Jouy-en-Josas, France homepage

References

Reviews

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Original publications

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