opuCB

glycine betaine/carnitine/choline/arsenobetaine/arsenocholine [SW|ABC transporter]

locus: BSU_33820

pI: 9.70

mw: 23.00 kDa

protein length: 217 aa

gene length: 654 bp

function: compatible solute transport

product: glycine betaine/carnitine/choline/arsenobetaine/arsenocholine [SW|ABC transporter]

essential: no

synonyms: yvbD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,469,184 3,469,837

The protein

Catalyzed reaction/ biological activity

• uptake of glycine betaine, arsenocholine and arsenobetaine [pubmed|29159878]

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
• [SW|CysTW subfamily] (according to UniProt)

Paralogous protein(s)

• [protein|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|OpuBB]

[SW|Domains]

• [SW|ABC transmembrane type-1 domain] (aa 19-198) (according to UniProt)

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]-[gene|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]-[gene|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]-[gene|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|opuCD]
  description: [Pubmed|10216873]

  regulatory mechanism

  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

  regulation

  • induced by salt stress [Pubmed|23960087,10216873]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• BKE33820 ([gene|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAAAAGAGCTCCTCC, downstream forward: _UP4_TCGTAAGGAGTTGGCACTGT
• BKK33820 ([gene|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAAAAGAGCTCCTCC, downstream forward: _UP4_TCGTAAGGAGTTGGCACTGT

References

Reviews

• 27935846

Original publications

• 10092453,9925583,10216873,21296969,23960087,23646920,29159878